Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Study of resistance to Toxoplasmosis using Tachizoites surface antigens of Toxoplasma gondii in vivo

Taoxoplasma gondii is an important food-borne parasite transmitted primarily from animals to humans through meat consumption, mainly pork and lamb, as well as through oocysts shed by cats. Infection in humans can cause severe fetal malformations, ocular complications or encephalitis. Toxoplasmosis infection during pregnancy, especially in sheep, often results in abortion, representing considera...

Evaluation of Recombinant SAG1 Protein for Detection of Toxoplasma gondii Specific Immunoglobulin M by ELISA Test

BACKGROUND Toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec-SAG1) for the detection of Toxoplasma-specific IgM antibodies in human sera, by an enzyme-linked immunosorbent...

Luminex® Assays High-throughput Multiplex Bead Based Assays

Panomics has an aggressive program for the development of a broad range of assays for the Luminex platform and similar instruments based on the xMAP technology. Currently we have hundreds of RNA targets available in 3–30 plex assays using our using our QuantiGene Plex Reagent Systems, with new targets being added weekly. Our Procarta® Human, Mouse and Rat Assays can quantitatively measure cytok...

Use of yeast-secreted in vivo biotinylated recombinant antibodies (Biobodies) in bead-based ELISA.

PURPOSE To measure circulating antigens, sandwich ELISA assays require two complementary affinity reagents. Mouse monoclonal antibodies (mAb) and polyclonal antibodies (pAb) are commonly used, but because their production is lengthy and costly, recombinant antibodies are emerging as an attractive alternative. EXPERIMENTAL DESIGN We developed a new class of recombinant antibodies called biobod...

High-level expression and evaluation of the antigenicity of a recombinant Toxoplasma gondii GRA2 Protein